Mammalian cells, such as CHO cells, are complicated factories for valuable products such as antibodies or vaccines. Timely monitoring and control are important tools in order to facilitate process robustness and product quality. An adequate and accepted reference tool for measurement of product, substrates, media components or metabolites are HPLC methods, facilitating physiological analysis of a process. Usually, sampling and sample processing is done manually, hence, sample analysis is done off-line at the end of the process. In this study, we want to show how this end-point measurements can be avoided as well as the benefits of automated and timely monitoring of processes.
Alexandra Hofer, Paul Kroll and Christoph Herwig, TU Wien, presented their research during BioTech 2017, Zürich