Innate NK Summit
Are you attending the digital Innate Killer Summit 2020?
Find out more about Ovizio’s holographic microscopy by visiting our booth in the virtual demo area or take a look at our joint poster with Medigen Biotechnology Corp: “Detecting morphology changes in activated NK cells using holographic fingerprinting”.
If you’re interested in more information, you can arrange a one-to-one meeting with our CEO, Emilie VIEY, PhD.
To register or learn more about the event, please visit: innate-killer.com
Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration
Animal Cell Technology Unit, iBET, Instituto de Biologia Experimental e Tecnológica
Daniel A. M. Pais , Paulo R. S. Galrão, Anastasiya Kryzhanska, Jérémie Barbau, Inês A. Isidro and Paula M. Alves*
This work shows that DHM can be implemented for online monitoring of Sf9 concentration and viability, as well as product titer (AAV) or culture progression in lytic systems. Therefore, it is a valuable tool to support the time of harvest decision and establishing controlled feeding strategies.
The insect cell-baculovirus vector system has become one of the favorite platforms for the expression of viral vectors for vaccination and gene therapy purposes. Because it is a lytic system, it is essential to balance maximum recombinant product expression with harvest time, minimizing product exposure to detrimental proteases. With this in mind, new bioprocess monitoring solutions are needed to accurately estimate culture progression. Herein, we used online digital holographic microscopy (DHM) to monitor bioreactor cultures of Sf9 insect cells