Priyal Patel and Jean-François Hamel, MIT, presented their results during the Bioprocessing Summit in Boston.

Mammalian cells, such as CHO cells, are complicated factories for valuable products such as antibodies or vaccines. Timely monitoring and control are important tools in order to facilitate process robustness and product quality. An adequate and accepted reference tool for measurement of product, substrates, media components or metabolites are HPLC methods, facilitating physiological analysis of a process. Usually, sampling and sample processing is done manually, hence, sample analysis is done off-line at the end of the process. In this study, we want to show how this end-point measurements can be avoided as well as the benefits of automated and timely monitoring of processes.

Alexandra Hofer, Paul Kroll and Christoph Herwig, TU Wien, presented their research during BioTech 2017, Zürich

At Ovizio, we are frequently asked why we chose double digital differential holography microscopy (D3HM) to monitor cell viability.
We thought that the most comprehensive way to approach an analytical procedure was to question every aspect of it as a validation
method.

We asked ourselves: What do we want to measure?

How can we measure it? How can this be achieved accurately and precisely?

What are the techniques involved? Which one is the best?

By reading the results of our study, you will get:

• A clear understanding of the meaning of accuracy and precision in cell viability
• An overview of the principles of the different methods, their advantages and limits
• An insight comparison of the techniques and instrument monitoring in cell viability in terms of their accuracy and precision

White paper available for download

Jérémie Barbau, Ovizio, presented the latest results during ESACT 2017 at Lausanne.